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Zymo Research
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Genomic DNA Clean & Concentrator-10
• Quick (5 minute) spin-column recovery of large-sized DNA (e.g., genomic,
mitochondrial, plasmid (BAC/PAC), viral, phage, (wga) DNA, etc.) from any enzymatic
reaction or impure preparation (e.g., Proteinase K digestion). No messy precipitations!
• Unique spin-column for low volume (≥10 μl) elution of ultra-pure, high-yield DNA.
• Eluted DNA is ideal for PCR, restriction endonuclease digestion, Sanger and Next-
Generation sequencing, etc.
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

HIGHLIGHTS

  • Quick, 5-minute clean-up of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations.
  • Unique spin column for low volume elution of ultra-pure, high-yield DNA.
  • Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc.
DESCRIPTION

The Genomic DNA Clean & Concentrator-10 (gDCC) is a gDNA clean up kit that provides quick, 5 minute recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, WGA DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied Zymo-Spin Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. This gDNA clean up kit is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.


Applicable ForEluted DNA is ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, restriction digestion procedures, and any other sensitive downstream application
Elution Volume≥ 10 µl of DNA Elution Buffer
EquipmentMicrocentrifuge
PurityA260/A280 > 1.8, A260/A230 > 1.8
Sample SourceEnzymatic reactions or impure preparations containing genomic DNA.
Sample StorageEluted DNA can be used immediately or stored at ≤ -20°C.
Size Range50 bp to 200 kb
YieldRecovery of DNA ranges from 70 - 95%





Q1: What is the lower limit and minimal amount of DNA that can be recovered?

Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.

Q2: How can I process naked DNA stored in DNA/RNA Shield?

Use the standard protocol (add 2 or 5 volumes of DNA binding buffer depending on DNA size).

Q3: What should I do if I did not add ethanol to the DNA Wash Buffer before starting?

The DNA will be eluted off the column during the wash step. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.

Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?

Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.

Q5: How many times can columns be reloaded?

Zymo Research recommends no more than 5 times, as binding efficiency might decrease.

Q6: What is the minimum input volume of DNA sample?

Working with volumes below 50 µl can result in decreased recovery. Zymo Research recommends raising the starting volume to 100 µl with water to ensure optimal binding conditions.




 

주문정보

CAT.No 품명 규격 비고
D4010 Genomic DNA Clean & Concentrator®-10 25 preps. Format: Spin Column Elution Volume: ≥ 10 μl Processing Time: 5 minutes Binding Capacity: 10 μg DNA Size Limit: 50 bp to ≥ 200 kb
D4011 Genomic DNA Clean & Concentrator®-10 100 preps. Format: Spin Column Elution Volume: ≥ 10 μl Processing Time: 5 minutes Binding Capacity: 10 μg DNA Size Limit: 50 bp to ≥ 200 kb