Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
• Quick (7 minute) protocol to select for ≥300 bp, ≥200 bp, ≥150 bp, ≥100 bp, ≥50 bp
DNA fragments or perform a double size selection.
• Clean and concentrate DNA from enzymatic reactions in as little as 10 μl of DNA/RNA
Free water.
• Eluted DNA is well suited for use in Next-Generation sequencing, PCR, DNA ligation,
endonuclease digestion, RT-PCR, etc.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
Quick and easy 7 minute spin-column procedure to select for ≥ 300 bp, ≥ 200 bp, ≥ 150 bp, ≥ 100 bp, ≥ 50 bp DNA fragments or perform a double size selection.
Clean and concentrate DNA from enzymatic reactions and library preparations in as little as 10 µl of nuclease free water.
Eluted DNA is well suited for use in next generation sequencing, PCR, DNA ligation, endonuclease digestion, etc.
Product Description
The Select-a-Size DNA Clean & Concentrator Kit (Select-a-Size DCC) is a size selection DNA kit that provides the quickest and easiest method for purifying a desired range of DNA fragments sizes from library preps, PCR, endonuclease digestions, ligations, etc. Simply adjust the binding conditions for the desired cutoff, bind, wash, and elute. Selectively recover ≥ 300 bp, ≥ 200 bp, ≥ 150 bp, ≥ 100 bp, ≥ 50 bp DNA fragments or perform a double size selection. Unique Zymo-Spin column technology yields high-quality DNA in just minutes that is suitable for next generation sequencing, PCR, and other downstream applications. The entire purification procedure can be performed in as little as 7 minutes for 2 preps or 20 minutes for 24 samples. (See figures below).
Technical Specifications
Applicable For
Next Generation sequencing, ligation reactions, PCR, labeling, and restriction endonuclease digestions.
Elution Volume
≥ 10 µl of DNA Elution Buffer
Equipment
Microcentrifuge
Purity
A260/A280 > 1.8, A260/A230 > 1.8
Sample Source
Purified or crude DNA samples.
Size Range
~50 bp to 23 kb. 50 bp, 100 bp, 150 bp, 200 bp, 300 bp separation points. Double size selection: > 700 bp.
The two beads differ in terms of their coated functional groups which also lends itself to varying binding and elution efficiency. Select-a-Size MagBeads MagBinding Beads bind both DNA and RNA, at around >100 bp cutoff. The addition of isopropanol to the procedure allows to bind fragments less than 100 bp. – smaller diameter of beads – take longer to magnetize/pellet, – slightly shorter drying time – Used in Select-a-Size MagBead Kit and RCC MagBead Kit, Quick 16S Library Prep Kit – much larger diameter – faster to magnetize – longer to drying time – Used in all other MagBead kits
