Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Quick (2 minute) desalting and recovery of ultra-pure DNA from enzymatic reactions (e.g., PCR and endonuclease digestions), cell-free lysates, etc.
Column design allows DNA to be eluted at high concentrations into minimal volumes.
Eluted DNA is well-suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
Quick (2 minute) desalting and recovery of ultra-pure DNA from enzymatic reactions (e.g., PCR and endonuclease digestions), cell-free lysates, etc.
Column design allows DNA to be eluted at high concentrations into minimal volumes.
Eluted DNA is well-suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
Product Description
The DNA Clean & Concentrator-25 (DCC-25) is a PCR purification kit designed for rapid desalting and purification of up to 25 µg DNA from enzymatic reactions (e.g., PCR), endonuclease digestions, or cell-free lysates. Simply add the specially formulated DNA Binding Buffer to your sample and transfer to the supplied Zymo-Spin column. This PCR purification kit features Zymo-Spin column technology to yield high-quality, purified DNA in just minutes, and it is compatible with cDNA and ssDNA. Eluted DNA is suitable for sequencing, microarray analysis, PCR, nucleotide blotting, and restriction endonuclease digestion procedures.
The only difference is the cap. Everything else between the columns (max capacity, column matrix, etc.) is the same. Unsure which to use? It mainly comes down to preference. Some people like the capped columns for easy labeling or added security while others like the uncapped columns for faster sample processing.
Working with volumes below 50 µl can result in decreased recovery. Zymo Research recommends raising the starting volume to 100 µl with water to ensure optimal binding conditions.
Add an equal volume of ethanol (95-100%) to the sample and mix well. The sample is ready-to-bind and does not require DNA Binding Buffer. Proceed to Step 2.
The DNA will be eluted off the column during the wash step. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.
We have a Zymoclean Gel DNA Recovery Kit that has chemistry compatible with agarose gel. However, if you have the DNA Clean & Concentrator kits on hand, you can clean up DNA from gel. You would need to purchase the Agarose Dissolving Buffer (Cat# D4001-1-50, D4001-1-100) separately, and follow the Zymoclean Gel DNA Recovery Kit protocol (link here).
