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Zyppy Plasmid Miniprep Kit

E.Coli 배양액에서 원심분리 없이 바로Lysis하여 간편한 고품질 Plasmid DNA 추출!

• The fastest, easiest kits available for high-throughput transfection-quality plasmid
DNA purification.
• Pellet-free procedure omits conventional cell pelleting and resuspension steps.
• DNA quality appropriate for cloning, sequencing, and transfection.

서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

HIGHLIGHTS

Fastest: 8 minutes from culture flask to high-quality plasmid DNA.
Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
High Quality: Ready for PCR, sequencing, cloning, and transfection.

DESCRIPTION

The Zyppy Plasmid Miniprep Kit is a plasmid DNA kit that features a pellet-free modified alkaline lysis system that bypasses the normal cell-pelleting and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify with Zymo-Spin column technology. Additionally, the patented colored buffers included in the kit allow for easy visualization of complete cell lysis and neutralization. The Zyppy Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is high quality, endotoxin-free, and well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.

Applicable For	Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.
Elution Volume	≥ 30 µl
Equipment	Microcentrifuge and/or a vacuum manifold.
Processing Time	8 min
Purity	Typical Abs260/280 ≥1.8.
Size Range	Up to 25 kb
Yield	Up to 25 µg of plasmid DNA per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.

Q1: I ran out of Zyppy Wash Buffer. Can I substitute it with a homemade solution or Wash Buffer from another kit?

Zyppy Wash Buffer is available for purchase, separately. However, you can substitute with DNA Wash Buffer, Plasmid Wash Buffer, or 80% ethanol.

Q2: I accidently left my Neutralization Buffer at room temperature. Will it be okay?

Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4 °C as soon as possible.

Q3: What are the endotoxin levels in plasmid DNA isolated with the Zyppy Kits?

≤ 50 Endotoxin Units/µg of plasmid DNA.

Q4: Can the Zyppy Plasmid kits be used to isolate large constructs (BAC/PAC)?

No, we recommend using our ZR BAC DNA Miniprep Kit or ZymoPURE II Kits for isolating large constructs.

Q5: Can the purified plasmid be transfected into eukaryotic cell lines?

Yes, all our plasmid preps have an endo-wash step to remove endotoxins and produce plasmid DNA that is ready for transfection with most cell lines.

Q6: What is the composition of the Zyppy Elution buffer?

10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.

Q7: Can E.coli grown in enriched growth media be used with this kit?

Yes, pellet and resuspend E.coli in water or TE before proceeding with the protocol.

주문정보

CAT.No	품명	규격	비고
D4036	Zyppy® Plasmid Miniprep Kit	50 preps.	Format: Spin-Column Sample Volume: 600 μl - 3 ml Processing Time: 8 minutes Elution Volume: ≥ 30 μl DNA Yield: ≤ 25 μg DNA Size Limits: ≤ 25 kb
D4019	Zyppy® Plasmid Miniprep Kit	100 preps.	Format: Spin-Column Sample Volume: 600 μl - 3 ml Processing Time: 8 minutes Elution Volume: ≥ 30 μl DNA Yield: ≤ 25 μg DNA Size Limits: ≤ 25 kb
D4020	Zyppy® Plasmid Miniprep Kit	400 preps.	Format: Spin-Column Sample Volume: 600 μl - 3 ml Processing Time: 8 minutes Elution Volume: ≥ 30 μl DNA Yield: ≤ 25 μg DNA Size Limits: ≤ 25 kb
D4037	Zyppy® Plasmid Miniprep Kit	800 preps.	Format: Spin-Column Sample Volume: 600 μl - 3 ml Processing Time: 8 minutes Elution Volume: ≥ 30 μl DNA Yield: ≤ 25 μg DNA Size Limits: ≤ 25 kb