Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
HIGHLIGHTS
Fast: 8x less hands-on time than conventional 16S library prep protocols.
Easy: Premixed Plate minimizes hands-on time and handling errors.
Simple Workflow: 100% Automation ready with only a single PCR step and no need for normalization. Just pool by equal volume!
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
DESCRIPTION
The Quick-16S Plus NGS Library Prep Kit (V4) is the fastest and simplest NGS library prep targeting the V4 region of the 16S rRNA gene for high-throughput sequencing. The automation-friendly protocol utilizes a premixed amplification plate and a single qPCR/PCR for combined targeted amplification and barcode addition. Pooling by equal volume and a single clean-up of the final library, rather than massive AMPure® bead-based clean-ups, simplifies the library prep process. Additional library quantification analysis such as TapeStation® analysis or gel electrophoresis are not necessary. With these features, the workflow dramatically reduces the hands-on time of library preparation to only 30 minutes.
Amplicon Size
The final amplicon size after 1-Step PCR (targeted amplification and barcode addition) is ~388 bp.
Equipment Needed (user provided)
Microcentrifuge, plate spinner (centrifuge), 96-well real-time quantitative PCR system (SYBR Green compatible recommended), or standard PCR system, and 96-well real-time PCR plates.
Index Sequences
10 bp indexes are listed in the MiSeq Sample Sheet Template provided.
Sample Input
Purified microbial DNA (≤100 ng), free of PCR inhibitors.
Sequencing Platform
Compatible with all Illumina® sequencing platforms using custom sequencing primers. We recommend the MiSeq® Reagent Kit v2 (300-cycle).
V4 Primer Sequences (adapters not included)
515f (GTGYCAGCMGCCGCGGTAA) and 806r (GGACTACNVGGGTWTCTAAT).
Purified total DNA from any organism free from PCR inhibitors. This system has been used by Zymo Research’s Microbiomics Services team to process samples from human, water, soil, food, plants, feces, biofilms, and much more.
The combination of using Equalase qPCR Premix to yield roughly equal amounts of libraries and a carefully curated barcode index list results in similar raw sequencing reads across samples.
Equalase amplification and the library prep design may cause some library products to not anneal well, causing a lack of a tight band. These libraries are perfectly fine because preparation for Illumina sequencing includes denaturing libraries into single strands.
This library has been designed to be sequenced using any 300-cycle reagent kit. The use of custom sequencing primers allows for a bit of overlap between reads when using Paired End sequencing, which improves the data quality.
