Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Highlights
Perform plasmid midipreps in only 16 minutes using a simple spin-column protocol.
Purify up to 1.2 mg of highly concentrated plasmid DNA directly from a spin-column.
Eluted plasmid DNA is Endotoxin-free and Transfection-Ready.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Plasmid Purification In Just 20 Minutes:
Highlights
Perform plasmid midipreps in only 16 minutes using a simple spin-column protocol.
Purify up to 1.2 mg of highly concentrated plasmid DNA directly from a spin-column.
Eluted plasmid DNA is Endotoxin-free and Transfection-Ready.
Product Description
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Midiprep kit provides the fastest and simplest method available to efficiently isolate up to 1.2 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a patented binding system, the ZymoPURE II Midiprep kit can process up to 50 ml of culture in less than 18 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is rapidly bound onto a column with either a vacuum or a centrifuge instead of a slow gravity flow column. Additionally, there is no alcohol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 6 µg/µl), endotoxin-free and transfection-ready. As an added convenience, the ZymoPURE II Plasmid Midiprep kit contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Transfection, transformation, lentivirus production, adenovirus production, AAV production, CRISPR, genome editing, in vivo studies, sequencing, restriction endonuclease digestion, in vitro transcription/translation, PCR, and other sensitive applications.
Binding Capacity
1.2 mg
Culture Input
≤ 50 ml
Elution Volume
≥ 150 µl
Endotoxin Levels
≤ 0.025 EU/µg of Plasmid DNA
Equipment
Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge
Format
Spin-Column
Processing Time
≤ 18 min
Purity
Typical Abs 260/280 ≥1.8 and Abs 260/230≥ 2.0
Size Range
Up to 200 kb
Yield
Up to 1.2 mg per preparation. Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized. Typical yields from 50 ml of overnight culture grown in LB for high copy number plasmids are 200 – 400 µg.
Yes, the standard protocol should work with other gram-negative bacteria. However, the user will need to validate the protocol for their particular species. For gram-positive bacteria, please refer to the Gram-Positive Bacteria Protocol in the Appendix of the kit protocol.
The vacuum pump should be a single or double-staged unit capable of producing at least 400 mm Hg pressure at the vacuum manifold. If less pressure is applied, centrifuge the column prior to washing to remove any residual lysate/buffer remaining in the matrix.
Yes, however, the pressure needs to be around 400 mm Hg for the liquid to pass through the columns quickly. Users should take caution, as the pressure of an in-house vacuum line can fluctuate drastically or be significantly reduced, depending on the demand in the building.
No, the ZymoPURE kits are only compatible with ZymoPURE Wash 2 and we cannot disclose a substitution due to the sensitive nature of the recipe. Additional ZymoPURE Wash 2 can be purchased separately.
Yes, however, special care should be taken throughout the entire protocol since there is more biomass. We recommend increasing the incubation time during lysis and centrifuging the neutralized lysate before loading onto the supplied syringe filter. Exceeding the recommended culture volume or using enriched media for high copy plasmids may overload and subsequently clog the columns, which can reduce DNA yield/quality or result in failed preps.
Yes, the standard ZymoPURE protocol has been successfully tested with constructs up to 200 kb. To increase the elution efficiency of large plasmid DNA, we recommend pre-warming the ZymoPURE Elution Buffer (50 ºC) and increasing the incubation time on the column up to 10 minutes prior to centrifugation.
Yes, the RNase A is fairly stable at room temperature, but we recommend placing it in 4 °C as soon as possible to ensure optimal performance throughout the life span of the product.
