ENGLISHHOMECONTACT USSITEMAP
Zymo Research
▪ HOME>제품정보>Zymo Research
Quick-16S/ITS NGS Library Prep Sampler
Highlights

The included Quick -16S/ITS™ Plus reactions produce automatically normalized libraries, regardless of sample input, and target a variety of bacterial 16S gene regions in addition to the fungal ITS2 region.

The included Quick -16S™ Full-Length Library Prep Kit reactions offer increased taxonomic resolution for samples sequenced on PacBio® & Oxford Nanopore Technologies® long-read platforms.

All full-size versions of the kits offered as part of the sampler are 100% automation ready with only a single PCR step, and support multiplexing of 96, 384, or 768 samples.
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

  • The included Quick -16S/ITS™ Plus reactions produce automatically normalized libraries, regardless of sample input, and target a variety of bacterial 16S gene regions in addition to the fungal ITS2 region.
  • The included Quick -16S™ Full-Length Library Prep Kit reactions offer increased taxonomic resolution for samples sequenced on PacBio® & Oxford Nanopore Technologies® long-read platforms.
  • All full-size versions of the kits offered as part of the sampler are 100% automation ready with only a single PCR step, and support multiplexing of 96, 384, or 768 samples.

Description

The Quick-16S/ITS™ NGS Library Prep Sampler provides 8 reactions from each of our Quick -16S/ITS™ Plus library prep kits targeting the V1-V2, V1-V3, V3-V4, V4, and fungal ITS2 hypervariable regions. Additionally provided are 8 reactions of the Quick -16S™ Full-Length Library Prep Kit, designed for usage on PacBio® & Oxford Nanopore® long read platforms to provide users unparalleled taxonomic resolution. Users can now explore all Microbiomics library prep kit offerings in a single, convenient sampling format ideally suited to parallel evaluation of several target regions.

Performance

Technical Specifications
Primer Sequences (adapters not shown)V3-V4: 341f(CCTACGGGDGGCWGCAG, CCTAYGGGGYGCWGCAG; 17 bp), 806r(GACTACNVGGGTMTCTAATCC; 24 bp)
ITS: ITS3f (GCATCGATGAAGAACGCAGC; 20 bp), ITS4r (TCCTCCGCTTATTGATATGC; 20 bp)
V4: 515f (GTGYCAGCMGCCGCGGTAA; 19 bp), 806r (GGACTACNVGGGTWTCTAAT; 20 bp)
V1-V2: 27f (AGRGTTYGATYMTGGCTCAG; 20 bp), 341r (CTGCWGCCHCCCGTAGG; 17 bp)
V1-V3: 27f (AGRGTTYGATYMTGGCTCAG; 20 bp), 515r (TBACCGCGGCTGCTGGCAC; 19 bp)
Full-Length 16S: 27f (AGRGTTYGATYMTGGCTCAG; 20 bp), 1492r (RGYTACCTTGTTACGACTT; 19 bp)
Amplicon SizesV3-V4: ~606 bp
ITS: ~480 bp
V4: ~388 bp
V1-V2: ~492 bp
V1-V3: ~650 bp
Full-Length 16S: ~1500 bp
Barcode Sequences10bp barcodes are used for all provided reactions. Barcode sequences are available for download in the 'Documents' section.
Index PrimersUnique dual indexes (barcodes) are used to label individual samples for all Quick-16S/ITSTM Plus reactions while combinational indexes are used for the Quick-16STM Full-Length Library Prep Kit.
Required EquipmentMicrocentrifuge, plate spinner (centrifuge), 96-well real-time quantitative PCR system (SYBR Green compatible) or standard PCR system, and 96-well real-time PCR plates.
Sample InputPurified microbial DNA ≤100 ng, free of PCR inhibitors.
Sequencing PlatformSequencing of V1-V2, V1-V3, V3-V4, and fungal ITS2 libraries require an Illumina Miseq/NextSeq. Sequencing of V4 libraries is possible on all Illumina platforms (with the use of custom sequencing primers). Full-length 16S libraries are compatible with both PacBio & Oxford Nanopore instruments.

Resources

Protocol: 
SDS (MSDS): 
Barcode Sequences: 
MiSeq Sample Sheet Template: 
PacBio Sample Sheet Template: 



주문정보

CAT.No 품명 규격 비고
D6499 Quick-16S/ITS NGS Library Prep Sampler 8 preps pre region