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Quick-DNA HMW MagBead Kit
Highlights
High Molecular Weight DNA: Extract high molecular weight DNA up to 150 kb from any sample.
Ultra-Pure: Highest DNA yield and purity equipped with RNA removal technology.
Third-Generation Sequencing Ready: Optimized for long read sequencing (including Oxford Nanopore™ and PacBio SMRT™ sequencing).
서한형 대리

Zymo Research 제품 담당자

경신과학(주)

영업부

H.P) 010-8832-6303

HanHyung Seo

Zymo Research Brand Manager

Kyongshin scientific Co., Ltd.

Sales Department

H.P) 82)10-8832-6303

제품소개

Highlights

  • High Molecular Weight DNA: Extract high molecular weight DNA up to 150 kb from any sample.
  • Ultra-Pure: Highest DNA yield and purity equipped with RNA removal technology.
  • Third-Generation Sequencing Ready: Optimized for long read sequencing (including Oxford Nanopore™ and PacBio SMRT™ sequencing).

Product Description


The Quick-DNA HMW Magbead Kit is the easiest method to purify high molecular weight DNA from any sample (including biological fluids, cell cultures, and solid tissues). The purified HMW DNA is ideal for long read sequencing and immediately ready for third-generation sequencing platforms such as Nanopore and PacBio SMRT Sequencing. With a unique binding system, the Quick-DNA HMW Magbead kit delivers ultra-pure and concentrated genomic DNA up to 150 kb.

For microbiome samples, we recommend using ZymoBIOMICS DNA Kits.

Technical Specifications

DNA PurityHigh quality DNA is ready for all sensitive downstream applications such as long read sequencing, PCR, endonuclease digestion, Southern blotting, genotyping, Next-Generation Sequencing, bisulfite conversion, etc. (A260/A230 ≥ 1.8).
DNA SizeCapable of recovering genomic and mitochondrial DNA sized fragments up to 150 kb. If present, plasmid, parasitic, microbial, and viral DNA will also be recovered.
DNA YieldThe DNA binding capacity is 10 µg per 50 µl MagBinding Beads used.
Elution Volume50 µl DNA Elution Buffer per 33 µl MagBinding Beads
EquipmentMagnetic rack, shaker and/or rotator, and microcentrifuge (optional)
Sample TypesAny cells, solid tissue, whole blood, saliva, biological fluids, buccal, swabs, samples stored in DNA/RNA Shield, etc.

Resources


Q1: What is the typical length of DNA extracted using this kit?

DNA profiles generated using this kit range from 20kb – 200kb depending on sample type and handling.

Q2: Has this kit been validated with Nanopore or PacBio SMRT sequencing?

Yes, you can directly input DNA extracted using this kit into any long-read sequencing workflow.

Q3: I need more than 10 µg of HMW DNA for my downstream application. Is there any way I can increase my yield?

To increase the amount of DNA recovered, simply increase the amount of MagBinding Beads and DNA Elution Buffer used in the system (e.g. 100 µl MagBinding Beads eluted in 150 µl DNA elution buffer can bind up to 20-25 µg of HMW DNA).

Q4: Can this kit be used with microbial or environmental samples?

ZymoBIOMICS DNA Kits are recommended for processing microbial and environmental samples for long-read metagenomics sequencing.

주문정보

CAT.No 품명 규격 비고
D6060 Quick-DNA HMW MagBead Kit 96 Preps