Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Highlights
Whole Genome Bisulfite (WGBS) library preparation in less than 4 hours and in one tube.
Reduced library preparation bias for accurate methylation calling
Reproducible genome coverage from any species (>90% of CpG sites detected in mammalian samples)
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
Whole Genome Bisulfite (WGBS) library preparation in less than 4 hours and in one tube.
Reduced library preparation bias for accurate methylation calling
Reproducible genome coverage from any species (>90% of CpG sites detected in mammalian samples)
Product Description
Zymo-Seq WGBS Library Kit is the only kit available for whole genome bisulfite (WGBS) library preparation in a single tube. Zymo-Seq WGBS Library Kit incorporates tagmentation technology to eliminate the tedious fragmentation, enzymatic, and clean-up steps required by conventional, ligation-based library preparation methods. This streamlined workflow minimizes hands-on time, making it an ideal choice for higher throughput applications. To prepare Zymo-Seq WGBS libraries, intact genomic DNA is first bisulfite converted. Then, the following library preparation procedures are completed in a single tube: (1) second-strand synthesis, (2) adapterization via tagmentation, and (3) library amplification and indexing. After purification, libraries are ready for sequencing on Illumina instruments.
WGBS is the gold standard for DNA methylation studies as it provides base-level methylation quantification for all cytosines. Zymo-Seq WGBS Library Kit is ideal for any species for whole-genome methylation calling of CG, CHG, and CHH sites. Over 90% of human and mouse CpG sites can be detected from Zymo-Seq libraries.
Technical Specifications
DNA Input
For optimal results, use 100 ng of high quality, intact genomic DNA as input. Protocol is compatible with inputs between 10 ng to 100 ng. DNA should be free of enzymatic inhibitors and RNA contamination. DNA can be resuspended in water, TE, or a low salt buffer.
Processing Time
~ 4 hours
Required Equipment
Microcentrifuge, thermal cycler with heated lid, magnetic separator, multichannel pipette (suggested)
Required material not provided
Illumina DNA Prep, (M) Tagmentation Kit (Illumina, Cat. No. 20060059 or 20060060)
Sequencing Platform
Libraries are compatible with all Illumina sequencing platforms (MiSeq, MiniSeq, HiSeq, NextSeq, NovaSeq)
No. The kit provides twelve (12) Unique Dual Index Primer Sets. If you require additional index primer sets, please contact our Technical Support for assistance (949) 679-1190 ext. 3 or tech@zymoresearch.com.
The size of the input genomic DNA is important for high-quality and high-yield library. DNA from FFPE samples are often highly degraded, so the quality of the library will be affected. Cell-free DNA are not compatible with the kit because the fragment size is too short for efficient tagmentation.
Zymo-Seq WGBS Library Kit is compatible with genomic DNA extracted from any species, whether it is mammalian, bacteria, or plants. It is best to use high-quality, intact genomic DNA that is RNA-free.
Libraries are suitable for any cycling number, but increased cycling numbers will require greater amount of adapter trimming. For most, 100 bp paired-end reads are suitable. The sequencing depth will be dependent on the genome size and coverage desired. Generally, aiming for 10x coverage per CpG site is recommended.
The number of reads (N) required for the desired coverage can be estimated by using the following equation: N = (Coverage)*(Diploid Genome Length)/Read Length. Bisulfite conversion reduces the complexity of the genome and have lower alignment rates compared to DNA-seq, so it is recommended to increase the number of reads by 25%. Therefore, for human WGBS at 10X coverage using 100 bp PE sequencing, at least 400 million reads are recommended.
Zymo-Seq WGBS kit is ideal for those working with higher inputs (10-100ng) of genomic DNA. It has minimal handling and clean-up steps, which makes it ideal for high-throughput library generation.
Pico Methyl-Seq would be a better option for those working with small DNA inputs (< 10ng) or highly fragmented DNA, such as cfDNA or FFPE DNA.
