Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Highlights
Simple workflow: Prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries in as little as 2 hours of hands-on time.
Low input: The only RRBS kit that produces NGS libraries from ≥ 10 ng of genomic DNA.
Accurate and reproducible: Unbiased methylation calling and reproducible CpG coverage.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
Simple workflow: Prepare Reduced Representation Bisulfite Sequencing (RRBS) libraries in as little as 2 hours of hands-on time.
Low input: The only RRBS kit that produces NGS libraries from ≥ 10 ng of genomic DNA.
Accurate and reproducible: Unbiased methylation calling and reproducible CpG coverage.
Product Description
Zymo-Seq RRBS Library Kit is an all-in-one RRBS library prep kit that generates NGS libraries with a simplified workflow for the classic method Reduced Representation Bisulfite Sequencing (RRBS). This all-in-one RRBS library prep kit allows for DNA methylation profiling at single-nucleotide resolution in CpG-rich regions of the genome. This Zymo-Seq RRBS Library Kit is the ideal RRBS library prep kit to generate libraries that cover a significant percent (≥70% if using human genomic DNA) of all CpG islands, promotors, and gene bodies while largely reduces the sequencing cost, making it ideal for high-throughput screening and comparison experiments.
Purified genomic DNA. The DNA should be free of enzymatic inhibitors and can be suspended in water, TE, or a low salt buffer. DNA with low 260/280 or 260/230 ratios should be purified prior to processing using the Genomic DNA Clean & Concentrator (Cat. No. D4010). This protocol is not recommended for samples from species with low CpG density.
Sequencing Platform Compatible
Libraries are compatible with all Illumina sequencing platforms except HiSeq X Series.
Yes. With this all-in-one kit, you can generate RRBS libraries with the genomic DNA of your interest. However, the reagents for library quantification and size distribution characterization are not provided and will need to be supplied by the customers and/or their sequencing service providers to ensure optimal practice for sequencing.
In principle, the Zymo-Seq RRBS Library Kit can be applied to genomic DNA from any species, as long as a reference genome exists for subsequent bioinformatic analysis and the genomic DNA is between 10 and 500 ng with high quality (a good rule of thumb for “high quality” is that the DNA is of little degradation and the majority of the content being higher than 10 kb).
It is possible to generate libraries with DNA extracted from FFPE samples. However, due to the degradation of such DNA, the quality of the libraries will be affected. Increasing the input amount may help but the quality of the libraries is not guaranteed.
We provide 12 pre-mixed Unique Dual Index (UDI) primer sets with the kits and therefore you can multiplex up to 12 libraries. If you want to multiplex more, please order the Zymo-Seq UDI Primer Plate (Indexes 1-96, Cat. No. D3096) separately.
The yield per library depends on the quality of the input genomic DNA and other factors. In general, the yield of each library is around 300 ng or more (if quantified with Nanodrop) when following the recommended PCR cycles in the instruction manual.
The optimal read length and sequence depth depend on the goal of the project and the subsequent bioinformatic analysis. In general, when handling mammalian samples, 50-bp single-end or paired-end sequencing should do as the RRBS libraries usually have comparatively short inserts. Longer read length is also possible if one wants to cover as many CpG sites as possible. Additionally, a 5-10x mean coverage per CpG site is recommended.
