Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Highlights
Ready to Use: Preassembled buffers allow for lightning-fast library preparation in as little as 4 hours without compromising quality.
Improved Performance: Prepare libraries with 7x less mitochondrial contamination, saving reads and increasing sequencing depth.
Outstanding Consistency: Produce highly correlated replicates from both fresh and frozen samples.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
Ready to Use: Preassembled buffers allow for lightning-fast library preparation in as little as 4 hours without compromising quality.
Improved Performance: Prepare libraries with 7x less mitochondrial contamination, saving reads and increasing sequencing depth.
Outstanding Consistency: Produce highly correlated replicates from both fresh and frozen samples.
Product Description
Zymo-Seq ATAC Library Kit provides a simple and streamlined workflow for Assay for Transposase Accessible Chromatin with high throughput Sequencing (ATAC-Seq) library preparation from mammalian cell and tissue input. This all-inclusive kit features an easy to follow protocol capable of preparing ATAC-Seq libraries from as little as 50,000 cells in as little as 4 hours with minimal hands on time.
Initial cell lysis is gentle, ensuring that while the important nuclei are spun out of the lysed cell and collected, the contaminating mitochondrial DNA stays within the cell and is discarded. Next, the transposase, preloaded with Illumina adaptors, simultaneously fragments and tags (tagments) open chromatin regions in one rapid reaction. Finally, the tagmented DNA is indexed and amplified via PCR with UDI Tag Primer Set, generating high quality libraries that are ready to be sequenced on any Illumina instrument.
Technical Specifications
DNA Input
For optimal results, use 50,000 cells per technical replicate. Cells should present viability (measure of the proportion of live cells within a population) >80%. Recommend using cell counter to measure viability accurately.
Equipment Required
Microcentrifuge, Biosafety cabinet, Cell counter, water bath, thermocycler, and thermoshaker with capacity to heat to 37°C and shake at 1000 rpm (We recommend USA Scientific Mixer HC™ with a thermo block that can fit 1.5 mL tubes and 2 mL Low Binding tubes).
Library Storage
Libraries eluted in DNA Elution Buffer may be stored at 4°C overnight or at -20°C for long term storage.
Processing Time
~ 4 hours
Reagents Not Provided
Trypan blue, PBS, DNase I, cell media, trypsin, FBS, and DMSO.
Sequencing Platform Compatibility
Libraries are compatible with Illumina sequencing platforms (HiSeq™, NextSeq™, NovaSeq™).
It is possible to generate libraries with inputs lower than 50k, however, the quality of such libraries is not guaranteed. Typically, we see more PCR duplicates in sequencing data as the amount of input decreases.
Each tube contains a premixed forward and reverse primer set that contain a unique i5 and i7 index, respectively. The concentrations of each UDI primer premix is 5µM total (2.5µM each primer).
Yes, however, the protocol is optimized for live mammalian cell input and adjustments will have to be made. Please contact (949)-679-1190 ext. 3 or tech@zymoresearch.com for technical support regarding your sample type.
