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Glyco-SPOT DNA Repair Assay Kit

Base Excision Repair (BER) is the predominant repair pathway responsible for removal of small lesions from DNA, like oxidized, alkylated, deaminated bases and abasic sites.
In humans, the mechanism of BER involves the initial action of DNA glycosylases...

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(010-8832-6303)

제품소개

SPOT-LX is based on a technology powered by LXRepair – spin-off of the CEA (French Alternative Energies and Atomic Energy Commission). This outstanding technology addresses the need to understand and characterize the DNA Repair network, a major cellular defence mechanisms against DNA damage. SPOT-LX assay allows defining a DNA Repair Enzyme Signature that quantify the functionality of various DNA Repair Enzymes.

DNA Repair enzymes contained in a biological sample react with series of lesion-containing DNA on a biochip. The repair reaction occurring at each lesion site is measured by fluorescence analysis.

Bertin Bioreagent offers pharmaceutical and cosmetic companies a tool for characterizing mechanisms of action of their molecules including the stages of research and clinical studies with an assay ready for use: the range of SPOT-LX assay kit.

Applications also include study of ageing, neurological diseases, oxidative stress, inflammation and exposure to genotoxics to identify specific biomarkers related to DNA Repair.

This DNA Repair Assay kit is based on multiplexed in vitro patented technologies (CEA and UJF under license) that measure DNA repair in micro-holder.

Size: 24 wells

Shipping: wet ice

Stability: Store at +4degrees; shelf life 6 months maximum after production

Specificity:

ubiquitus for all species

Application Media: The assay can be conducted with whole cell extracts, nuclear extracts, tissue extracts or purified enzymes.

Custom Code: 38220000

UNSPSC code: 41116104

Garreau-Balandier I, et al. A comprehensive approach to determining BER capacities and their change with aging in Drosophila melanogaster mitochondria by oligonucleotide microarray. FEBS Lett. 2014; 588:1673-9.

Candéias S, et al. Direct inhibition of excision/synthesis DNA repair activities by cadmium: analysis on dedicated Biochips. Mutat Res. 2010; 10:53-9

Pons B, et al. Age-associated modifi cations of Base Excision Repair activities in human skin fi broblast extracts. Mech Ageing Dev. 2010;131:661-5

Guerniou V, et al. Repair of oxidative damage of thymine by HeLa whole-cell extracts: simultaneous analysis using a microsupport and comparison with traditional PAGE analysis. Biochimie. 2005; 87:151-9.

Sauvaigo S, et al. An oligonucleotide microarray for the monitoring of repair enzyme activity toward different DNA base damage. Anal Biochem. 2004; 333:182-92

주문정보

CAT.No	품명	규격	비고
LX05001	Glyco-SPOT DNA Repair Assay Kit	24 wells	Miscellaneous