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ELISA Training Kit
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...
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Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay
Product Overview: 
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
The kit B05005 has been designed to be used by 24 students and to perform 4 samples in duplicate. The kit is also available in half-size format for evaluation purposes under the reference B05006. The training EIA kit specifically measures the bovine β-lactoglobulin based on a double antibody sandwich technique.


Size: 2 x 96 wells
Shipping: room temp.
Stability: Store at multiT° RT/+4degrees; shelf life 3 years maximum after production
Tracer: AcetylCholinesterase AChE
Custom Code: 3822000000
UNSPSC code: 41116104


PRODUCT EXPLANATION: ACETYLCHOLINESTERASE MARKER IN ENZYME-IMMUNOASSAYS

Grassi J., Pradelles P. Compounds labelled by the acetylcholinesterase of Electrophorus Electricus. Its preparation process and its use as a tracer or marquer in enzymo-immunological determinations. United States patent, N° 1,047,330. September 10, 1991

Grassi J., Pradelles P. The use of Acetylcholinesterase as a Universal marker in Enzyme-Immunoassays. Proceedings of the Third International Meeting on Cholinesterases, American Chemical Society (1991)

Pradelles P., Grassi J., Maclouf J. Enzyme Immunoassays of Eicosanoids Using Acetylcholinesterase. Methods in Enzymology (1990), vol. 187, 24-34

PRODUCT EXPLANATION: TRAINING KIT DEVELOPMENT

Negroni L, Bernard H, Clement G, Chatel J.M, Brune P, Frobert Y, Wal J.M, Grassi J. Two-site enzyme immunometric assays for determination of native and denatured β-lactoglobulin. Journal of Immunological Methods 200 (1998), 25-37

Venien A, Levieux D, Astier C., Briand L, Chobert JM, Haertle T. Production and Epitopic Characterization of Monoclonal Antibodies Against Bovine β-Lactoglobulin. Dairy Sci (1997), 80:1977-1987

주문정보

CAT.No 품명 규격 비고
B05005 ELISA Training Kit 2 x 96 wells AChE kits