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IL-17A (pig) ELISA kit

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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(010-8832-6303)

제품소개

Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay

Synonyms:

Interleukin-17A

Correlated keywords:

ELISA

Chemokine

Paneth cells

NK cells

proinflammatory

Interleukin-17A

Th17

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Interleukin-17A (IL-17A) is one of the six members of the IL-17 family(1) (from IL-17A to IL-17F).
IL-17A is a polypeptide of 132 amino acids with a molecular weight of 14.9 kDa. IL-17A structure contains 4 antiparallel strands connected by two disulfides bridges(2). It can form either a homodimer or a heterodimer with IL-17F.
Cells from the lymphoid lineage (CD4+ T cells, Th17 subpopulation, NK cells)(3-4) and Paneth cells(5) have been identified as producers of IL-17A.
IL-17A is a proinflammatory mediator, its upregulation is find in many diseases like skin disorders (psoriasis, acne inversa) or Crohn's disease.

Size: 96 wells

Shipping: wet ice

Stability: Store at +4degrees; shelf life 6months maximum after production

Specificity:

refer to technical booklet

Application Media: It is the responsibility of the user to check the compatibility of the assay with the study matrix

Sample volume: 100 µL

Tracer: horseradish peroxidase HRP

Detection Limit: ≤0,02 ng/mL

Standard Curve Range: 0,02-2,00 ng/mL

Custom Code: 3822000000

UNSPSC code: 41116104

1. Iwakura Y, Ishigame H, Saijo S, Nakae S Functional specialization of interleukin-17 family members. 2011, Immunity 34:149–162

2. Robert Sabat, Ellen Witte, Katrin Witte, and Kerstin Wolk IL-22 and IL-17: An Overview 2013, IL-17, IL-22 and Their Producing Cells: Role in Inflammation and Autoimmunity, 2nd ed., pp.11-35

3. Harrington LE, Hatton RD, Mangan PR et al. Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages. 2005, Nat Immunol 6:1123–1132

4. Moreira-Teixeira L, Resende M, Coffre M, Devergne O et al. Proinflammatory environment dictates the IL-17-producing capacity of human invariant NKT cells. 2011, J Immunol 186:5758–5765

5. Takahashi N, Vanlaere I, de Rycke R, Cauwels A, Joosten LA, Lubberts E, van den Berg WB, Libert C IL-17 produced by Paneth cells drives TNF-induced shock. 2008, J Exp Med 205:1755–1761

주문정보

CAT.No	품명	규격	비고
A05423	IL-17A (pig) ELISA kit	96 wells	HRP kits