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IL-13 (pig) ELISA kit

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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(010-8832-6303)

제품소개

Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay

Synonyms:

Interleukin-3

Correlated keywords:

ELISA

Chemokine

IL-4

T helper cell subsets CD8+ T

mast cells

eosinophils

basophils

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
Interleukin 13 (IL-13) is a nonglycosylated cytokine of 132 amino acids with a molecular weight of 12kDa and structured with four alpha-helices(1). IL-13 is secreted by T helper cell subsets, CD8+ T cells, mast cells, eosinophils and basophils(3).
It has 25 % of sequence homology with IL-4(1), they share their actions on mononuclear phagocytic cells, endothelial cells, epithelial cells, and B cells(4). Both are key factors in the development of allergic hypersensitivity, and they also play a major role in exacerbating allergic and asthmatic symptoms.
IL-13 is more importantly implicated as a central mediator of the physiologic changes induced by allergic inflammation in many tissues(2). IL-13 is the central mediator of allergic asthma, where it regulates eosinophilic inflammation, mucus secretion, and airway hyperresponsiveness(4). It's could play role in the resistance of some intracellular parasitis by down regulated of pro-inflammatory cytokines.

Size: 96 wells

Shipping: wet ice

Stability: Store at +4degrees; shelf life 6months maximum after production

Specificity:

refer to technical booklet

Application Media: It is the responsibility of the user to check the compatibility of the assay with the study matrix

Sample volume: 100 µL

Tracer: horseradish peroxidase HRP

Detection Limit: ≤0,02 ng/mL

Standard Curve Range: 0,02-2,00 ng/mL

Custom Code: 3822000000

UNSPSC code: 41116104

1. Juha Punnonen, José M. Carballido, Gregorio Aversa, Jan E. de Vries Interleukin 13 and its Receptor 1998, Encyclopedia of Immunology (Second Edition) Pages 1489–1492

2. Minty A, Chalon P, Derocq JM et al. Interleukin-13 is a new human lymphokine regulating inflammatory and immune responses March 1993, Nature 362 (6417): 248–50.

3. Lakshmi Srinivasan, Mary Catherine Harris, Laurie E. Kilpatrick Cytokines and Inflammatory Response in the Fetus and Neonate 2017, Fetal and Neonatal Physiology (Fifth Edition), 128: Pages 1241–1254

4. Terufumi Kubo, Hideaki Morita, Kazunari Sugita, Cezmi A. Akdis Chapter 1 – Introduction to Mechanisms of Allergic Diseases 2017, Middleton's Allergy Essentials Pages 1–27

주문정보

CAT.No	품명	규격	비고
A05422	IL-13 (pig) ELISA kit	96 wells	HRP kits