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IL-6 (pig) ELISA kit

Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme wh...

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(010-8832-6303)

제품소개

Territorial Availability: Available worldwide directly through Bertin Technologies or your local distributor

Technical Warning: Check the Additional Items Required section of this kit booklet to verify if UltraPure Water (Milli-Q or equivalent) is needed for this assay

Synonyms:

Interleukin-6

Correlated keywords:

ELISA

Cytokine

Inflammatory

Diabetes

Cancer

TNF-alpha

Interleukin-11

Product Overview:
Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).
Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.
IL-6 is a protein of 212 amino acids. It’s a pleiotropic cytokine which has an impact on the regulation of immune and nonimmune events. It is produced by the monocytes and macrophages in response to other infl ammatory cytokines like Interleukin-11 (IL-11) and the Tumor Necrosis Factor-beta (TNF-β).
IL-6 acts on immune and nonimmune cells: it helps to differentiate T-cells from B-cells but it is also vital for the development of blood cells (white cells, red cells or platelets).
Due to these target cells, IL-6 is an activator of immune system and infl ammation. It also controls the metabolism by action on adipocytes, the bone metabolism and the pain.
A dysregulation in IL-6 production induces many disorders such as autoimmune diseases, infl ammation diabetes or cancer.

Size: 96 wells

Shipping: wet ice

Stability: Store at +4degrees; shelf life 6 months maximum after production

Specificity:

refer to technical booklet

Application Media: It is the responsibility of the user to check the compatibility of the assay with the study matrix

Sample volume: 100 µL

Tracer: horseradish peroxidase HRP

Detection Limit: ≤0,08 ng/mL

Standard Curve Range: 0,08-10,00 ng/mL

Custom Code: 3822000000

UNSPSC code: 41116104

Toshio T., Masashi N., Tadamitsu K. IL-6 in Infl ammation, Immunity, and Disease. Cold Spring Harb Perspect Biol. 2014 Sep 4;6(10)

Kristiansen O.P. , Mandrup-Poulsen T. Interleukin-6 and Diabetes The Good, the Bad, or the Indifferent? Diabetes, 54 (2005) Supp. 2: S114-124

Scheller J., Chalaris A., Schmidt-Arras D. and al. The pro- and anti-infl ammatory properties of the cytokine interleukin-6. Biochimica et Biophysica Acta 1813 (2011) 878–888

Skurk T., Alberti-Huber C., Herde C., and al. Relationship between Adipocyte Size and Adipokine Expression and Secretion. J Clin. Endocrinol. Metab. 92 (3) (2007) 1023–1033

주문정보

CAT.No	품명	규격	비고
A05410	IL-6 (pig) ELISA kit	96 wells	HRP kits