Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
The simplest method for generating random-ended dsDNA fragments.
Fragment size is conveniently controlled by adjusting the enzyme concentration.
dsDNA Shearase™ Plus-generated fragments are ideal for library construction, Next-Gen sequencing, and methylated DNA immunoprecipitation (MeDIP).
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
HIGHLIGHTS
The simplest method for generating random-ended dsDNA fragments.
Fragment size can be controlled by adjusting the enzyme concentration.
Fragments generated using dsDNA Shearase Plus are ideal for library construction, Next-Gen sequencing, methylated DNA immunopreciptation (MeDIP, MeDIP-Seq), etc.
DESCRIPTION
dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. It has a particularly strong preference for dsDNA and generates random-ended DNA fragments of the desired size in a single step. This enzyme is compatible with low volume inputs, thus minimizing sample loss.
Concentration
1 U/µl
Enzyme Inactivation
Heat inactivate enzyme at 65°C for 5 min.
Storage
Store at -20°C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70°C.
Unit Definition
One unit (1 U) is defined at the amount of enzyme required to convert 250 ng human DNA into DNA fragments in the range of 100-500 bp in 20 minutes at 42°C in total reaction volume in 10 µl.
주문정보
CAT.No
품명
규격
비고
E2018-50
dsDNA Shearase™ Plus
50 U
Provided with 5X dsDNA Shearase™ Plus Reaction Buffer
E2018-200
dsDNA Shearase™ Plus
200 U
Provided with 5X dsDNA Shearase™ Plus Reaction Buffer
