Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Highlights
Quick and simple procedure for generating single nucleosides from DNA for quantitative analysis via LC/MS
Convienent 2 hour, single enzyme digest v. conventional 6-16 hr multi-step enzyme digestion protocol
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
Quick and simple procedure for generating single nucleosides from DNA for quantitative analysis via LC/MS
Convienent 2 hour, single enzyme digest v. conventional 6-16 hr multi-step enzyme digestion protocol
Product Description
DNA Degradase Plus from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleoside components. Since nucleosides lack negatively charged phosphate, DNA Degradase Plus is ideal for whole-genome DNA methylation analysis by LC/MS. Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.
Technical Specifications
Assay Condition
DNA Degradase Plus in 1X DNA Degradase Reaction Buffer. Incubate reaction mixtures at 37C for ≥1 hour
Concentration
5 U/µl
Enzyme Inactivation
Heat inactivate enzyme at 70C for 20 minutes
Storage
Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70C.
Unit Definition
5 U of enzyme will digest 1 µg of genomic DNA in 25 µl reaction volume at 37C for ≥ 1 hr
The best way to confirm degradation is to run the sample on a gel. Nothing should be visible for the Degradase-treated samples. You do not need to purify the reaction.
Yes, the protocol time is a suggestion for sufficient digestion. Additional incubation time can be added to completely ensure that all sample has been digested. There is no harm in letting the reaction proceed longer.
The preferred substrate for Degradase and Degradase Plus is double stranded DNA. There will be only minor degradation of single stranded DNA template and no degradation of RNA template.
Yes, you can scale the reaction up or down as necessary. For the best results, digest no more than 1 µg of DNA with 5 U (1 µl) of enzyme in 25 µl reaction volume. The reaction volume is just as important as the amount of DNA, and we recommend scaling up the volume of enzyme accordingly. For example, if the reaction volume is 100 µl, use 4 µl of DNA Degradase (Plus).
