Zymo Research
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DNA Degradase Plus
HIGHLIGHTS
▶Quick and simple procedure for generating single nucleosides from DNA for quantitative analysis via LC/MS
▶Convienent 2 hour, single enzyme digest v. conventional 6-16 hr multi-step enzyme digestion protocol
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
DESCRIPTION
DNA Degradase Plus from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleoside components. Since nucleosides lack negatively charged phosphate, DNA Degradase Plus is ideal for whole-genome DNA methylation analysis by LC/MS. Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.
| Assay Condition | DNA Degradase Plus in 1X DNA Degradase Reaction Buffer. Incubate reaction mixtures at 37C for ≥1 hour |
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| Concentration | 5 U/µl |
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| Enzyme Inactivation | Heat inactivate enzyme at 70C for 20 minutes |
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| Storage | Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70C. |
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| Unit Definition | 5 U of enzyme will digest 1 µg of genomic DNA in 25 µl reaction volume at 37C for ≥ 1 hr |
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주문정보
| CAT.No |
품명 |
규격 |
비고 |
| E2020 |
DNA Degradase Plus™ |
250 U |
Provided in solution (10 units/µl) w/ 10X Reaction Buffer |
| E2021 |
DNA Degradase Plus™ |
1,000 U |
Provided in solution (10 units/µl) w/ 10X Reaction Buffer |
