Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Highlights
High-throughput purification of high quality, inhibitor-free DNA from any sample including feces, soil, water, biofilms, swabs, saliva, and body fluids.
The ZymoBIOMICS innovative lysis system enables efficient and unbiased lysis of microbes including Gram-positive/negative bacteria, fungi, protozoans, algae, etc.
The automation friendly workflow enables nearly any sample to be processed in as little as 90 minutes for 96 preps.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
High-throughput purification of high quality, inhibitor-free DNA from any sample including feces, soil, water, biofilms, swabs, saliva, and body fluids.
The ZymoBIOMICS innovative lysis system enables efficient and unbiased lysis of microbes including Gram-positive/negative bacteria, fungi, protozoans, algae, etc.
The automation friendly workflow enables nearly any sample to be processed in as little as 90 minutes for 96 preps.
Product Description
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume
≥ 37.5 µl DNA Elution Buffer or DNase free water
Equipment
Centrifuge fitted with a 96 well microplate carrier, 96 Well Magnetic Stand, Liquid handler or other robotic sample processor, 96 well plate heat block
High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing
Sample Source
Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water.
Sample Storage
Eluted DNA should be stored at ≤ -20°C.
Size Range
Typically 15-20 kb post-bead beating. For optimal DNA integrity, collect samples in DNA/RNA Shield.
Type
Total DNA
Yield
Up to 10 µg total DNA can be eluted into 50 µl (37.5 µl minimum)
Yes, we currently have scripts for Hamilton and KingFisher, as well as support for Tecan on some systems. Please contact product specialist for specific inquiries.
Yes, our protocol includes an “automation guide” for users to script the protocol themselves and our technical support staff also provides troubleshooting and advice if needed.
Check sample type and input amount. Low biomass samples such as swabs, some soil samples, water, air are expected to have low yield. High biomass samples such as feces may cause overloading of the spin column. Using less input may improve yields.
Ensure sample is fully homogenized. Depending on bead beater used, the settings may have to be optimized for each sample. The homogenization time may be extended to see if yields improve.
Apply heated elution buffer (60-70° C) and allow elution buffer to incubate on the column for several minutes prior to elution.
Ensure that the DNA Elution Buffer is added directly to the column matrix.
Low A260/230 values can be due to handling issues (e.g. transfer of column during wash steps). To minimize reagent wash buffer carryover, centrifuge the column at max speed for 1 minute prior to elution.
주문정보
CAT.No
품명
규격
비고
D4302
ZymoBIOMICS 96 MagBead DNA Kit (Lysis Rack)
2 x 96 preps
D4306
ZymoBIOMICS 96 MagBead DNA Kit (No Lysis Matrix)
2 x 96 preps
D4308
ZymoBIOMICS 96 MagBead DNA Kit (Lysis Tubes)
2 x 96 preps
D4302-E
ZymoBIOMICS 96 MagBead DNA Kit – Dx (Lysis Rack)
2 x 96 preps
D4306-E
ZymoBIOMICS 96 MagBead DNA Kit – Dx (No Lysis Matrix)
