Zymo Research는 1994년도에 설립된 미국 회사로 고품질 키트 제조회사 입니다.
각종 샘플(세포, 조직, 환경샘플 등)로부터 고품질
DNA나 RNA를 가장 쉽고 빠르게 뽑을 수 있습니다.
이 외에도, 각종 Epigenetics 관련 제품들
(DNA Methylation kit 등)과 Microbiomics
(샘플 채집부터 분석까지의 전 단계의 제품)
연관 제품들이 준비되어 있습니다.
Exceptional Stability: Optimized novel formulation for superior stability.
Batch-to-Batch Consistency: Enables highly reproducible assays and library preps.
Ready-to-Load: Easily generate transposomes or load with custom adapters with Unloaded format.
Ready-to-Tagment: Immediately tagment DNA for short-read sequencing library prep with Loaded format.
서한형 대리
Zymo Research 제품 담당자
경신과학(주)
영업부
H.P) 010-8832-6303
HanHyung Seo
Zymo Research Brand Manager
Kyongshin scientific Co., Ltd.
Sales Department
H.P) 82)10-8832-6303
제품소개
Highlights
Exceptional Stability: Optimized novel formulation for superior stability.
Batch-to-Batch Consistency: Enables highly reproducible assays and library preps.
Ready-to-Load: Easily generate transposomes or load with custom adapters with Unloaded format.
Ready-to-Tagment: Immediately tagment DNA for short-read sequencing library prep with Loaded format.
Product Description
EnduraTM Tn5 Transposase is hyperactive mutant of Tn5 Transposase with a proprietary formulation to confer superior stability, efficient tagmentation, and reliable consistency between experiments. EnduraTM Tn5 Transposase allows for easy, rapid, and consistent library preparation for next-generation libraries such as whole genome sequencing, shotgun sequencing, targeted sequencing, single-cell sequencing, and ATAC-Seq.
Applications
Whole Genome Sequencing (WGS) – Decode the entire DNA sequence of an organism’s genome to identify genetic variants, mutations, and structural variations.
Whole Exome Sequencing (WES) – Analyze the protein-coding regions of the genome to detect mutations linked to particular phenotypes, genetic disorders, and disease.
Assay for Transposase-Accessible Chromatin Sequence (ATAC-Seq) – Identify genome-wide chromatin accessibility and regulatory DNA elements in high resolution.
Targeted Sequencing – Focus on specific genes or genomic regions to detect known mutations, SNPs, and disease-related variants with accuracy.
Epigenomic Sequencing – Map DNA methylation and histone modifications to study gene expression regulation and epigenetic changes.
Metagenomic Sequencing – Analyze microbial DNA from environmental or host-associated samples to profile microbial diversity, taxonomy, and functional genes.
Single Cell Sequencing – Analyze gene expression, epigenetics, or genome variation at the individual cell level to uncover cellular heterogeneity.
RNA Sequencing – Capture and quantify the transcriptome to analyze gene expression, alternative splicing, and non-coding RNA profiles.
Transposome Studies – Examine transposable elements and mobile DNA to understand their role in genome evolution, stability, and regulation.
Technical Specifications
Activity
Tn5 Transposase (1 U/µl)
Format
Liquid, 50% glycerol
Concentration
~12.5 µM, ~1 mg/ml
Optimum pH
7.5
Optimum Temperature
55°C
Storage
Store at -20°C, For long term storage, store at -80°C.
Endura TM Tn5 Transposase is a hyperactive variant of Tn5 transposase with a novel formulation to confer superior stability. It can maintain its activity over many freeze-thaw cycles as well as incubation at room temperature.
Endura TM Tn5 Transposase can be inactivated by addition of 10x Stop Buffer to a final concentration of 1x in your reaction, following by a 5 minute incubation at 75 °C.
Yes, you can load Endura TM Tn5 Transposase with any custom adapter sequence, of any length, as long as the 3’ end contains 19-bp Mosaic-End sequence (5’ – CTGTCTCTTATACACATCT – 3’).
